p144505  (Toronto Research Chemicals)

Journal: iScience

Article Title: Insulin acts on astrocytes to shift their substrate preference to fatty acids

doi: 10.1016/j.isci.2024.111642

Figure Lengend Snippet:

Article Snippet: 13 C 16 palmitate , Toronto Research Chemicals , P144505.

Techniques: Recombinant, Software

Journal: iScience

Article Title: Insulin acts on astrocytes to shift their substrate preference to fatty acids

doi: 10.1016/j.isci.2024.111642

Figure Lengend Snippet:

Article Snippet: 13 C 16 palmitate , Toronto Research Chemicals , P144505.

Techniques: Recombinant, Software

Journal: Cancers

Article Title: PI3K/AKT/β-Catenin Signaling Regulates Vestigial-Like 1 Which Predicts Poor Prognosis and Enhances Malignant Phenotype in Gastric Cancer

doi: 10.3390/cancers11121923

Figure Lengend Snippet: VGLL1 expression is regulated by the PI3K/Akt/β-catenin pathway. ( a ) Expression of VGLL1 mRNA after treatment with si PIK3CA or si PIK3CB . ( b ) NUGC3 cells incubated with inhibitors (10 µM) for 24 h. The mRNA and protein expression of VGLL1 was analyzed by RT-PCR and western blotting, respectively. ( c ) Effects of LY294002 on VGLL1 expression in gastric cancer cells. ( d ) mRNA levels of VGLL1 , VGLL4 , and YAP in NUGC3 cells treated with LY294002 and analyzed by RT-PCR. ( e ) NUGC3 cells incubated with LY294002 for 6 h at the indicated concentrations. Expression of the proteins was analyzed by western blotting. ( f ) Effect of AKT on VGLL1 expression assessed by western blotting. NUGC3 cells expressing wild-type (WT) AKT or constitutively active (CA) AKT were treated with LY294002. ( g ) Localization of β-catenin to the VGLL1 promoter. ChIP assay was performed using nuclear extracts of NUGC3 cells treated with LY294002. The ChIP-enriched DNA was subjected to PCR. ( h ) Effect of β-catenin expression knockdown or overexpression on VGLL1 expression in NUGC3 cells analyzed by RT-PCR and western blotting. ( i ) VGLL1 -promoter-driven luciferase reporter activity. NUGC3 cells were treated with siRNAs and then transfected with VGLL1-luc and Renilla-luc vectors. ( j ) Effect of LY294002 on VGLL1 promoter activity. NUGC3 cells were co-transfected with β-catenin, p300 , TCF4 , LEF1 , VGLL1 -luc, and Renilla-luc for 24 h and then incubated with 10 µM LY294002 for 24 h. Mean ±SD of three independent experiments with triplicate measurements.* p < 0.05, ** p < 0.01, *** p < 0.005 (Student’s t -test). ( k ) The effects of β-catenin, p300, TCF4, and LEF1 on VGLL1 expression, assessed by western blotting.

Article Snippet: The primers of PIK3CB (P144505) was purchased from Bioneer (Daejeon, Korea).

Techniques: Expressing, Incubation, Reverse Transcription Polymerase Chain Reaction, Western Blot, Knockdown, Over Expression, Luciferase, Activity Assay, Transfection